| Download Contract Agreement File: DOC File (Microsoft word) PDF File Download, print, fill out and then fax or mail it to us. Fax is preferred. |
| Development of hybridoma cell lines includes three phases. Viro Dynamics will consult with customers at each phase. In order to minimize the expenses for our customers, a project can be stopped at any Phase on consultation with Viro Dynamics Corp Phase A. Immunization and Bleeding of Eight Mice Mice and serum screening by ELISA . . . . . . . . . . . . . $1,500 Eight mice (BALB/cAnNHsd) are immunized with antigen provided by customer according to our Standard Protocol for Immunization of Mice for Preparation of Hybridoma lines. Serum is screened for titer by ELISA. This phase takes four to six weeks. Phase B. Fusion and Screening of Hybridoma Supernatant Price is for both Phase A and B . . . . . . . . . . . . . . . . . $5,000 Upon successful completion of Phase A, selected mice will be euthanized and the spleens harvested. A fusion procedure will be performed and the cells will be distributed into six or eight multiwell plates (96 well) per spleen. Screening will be performed by ELISA using previously supplied antigen. If after the first fusion attempt, no acceptable development is observed, additional attempts will be made using remaining mice from Phase A at no additional charge. This phase takes six to eight weeks. Phase C. Cloning of Parental Hybridomas Price is for Phase A, Phase B, and Phase C . . . . . . . $7,500 One to five of the parental hybridomas from Phase B will be selected, cloned, and screened by ELISA. Clones with the desired titer and specificity will be expanded and cryopreserved. At least five clones will be provided and delivered to the customer and/or we can start In Vitro Monoclonal Antibody Production. This phase takes four to six weeks. Before starting Phase A, a down-payment of $3,750 is required. The customer may stop the project after Phase A or Phase B for any reason. $2,250 will be refunded if project is terminated after Phase A. $1,250 will be invoiced if the project is terminated after Phase B. |
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| Production of Hybridoma Lines & Custom Monoclonal Antibodies |
