Viro Dynamics offers complete Immunochemistry services, from antigen development
to antibody purification and packaging.

Conjugation of Antigen
Conjugation to KLH, BSA, OVA. Other carriers also available.
Terminal modifications. C-Terminal amide formation and N-Terminal acetylation.

Purification of IgG
IgG will be purified from serum or ascites fluid using protein G chromatography. Protein
concentration will be determined. Final yield of purified antibodies depends on titer.

Affinity Purification of Antibodies
PURIFICATION OF ANTIBODIES FROM 10-20ML SERUM
Covalently link peptide or protein (1-2mg) to column through amino groups

Antibody Titration by ELISA
TITRATION ASSAY IN 96 WELL MICROPLATE
ASSAY PERFORMED IN DUPLICATE
FOUR SERA CAN BE TITERED PER PLATE
10 to 20 ug antigen needed per plate for coating
protein concentration must be provided
need to know buffer conditions (any detergent)

Western Blot Assay
FOUR LANES ASSAYED
need to supply 2 to 5 ug/lane
protein concentration must be provided
need to know buffer conditions (any detergent)
approximate molecular weight of protein of interest

SDS Polyacrylamide Gel Electophoresis
FOUR LANES ASSAYED
SAMPLE RUN UNDER REDUCING CONDITIONS
COOMASSIE STAINED GELS
need to supply 50 to 100 ug/lane
protein concentration must be provided
need to know buffer conditions (any detergent)
approximate molecular weight of protein of interest

Purification of IgY
IgY will be purified from twelve eggs by polyethylene glycol precipitation. Protein
concentration will be determined.
Immunochemistry Services
Information Request
I am interested in:
My name is:
My telephone number:
My postal address:
My E-mail address: